![]() Using a commercial DNA isolation kit, circulating cell-free DNA (ccfDNA) was extracted from the maternal plasma samples. Maternal plasma was separated from whole blood via centrifugation. ![]() Three to four milliliters (mL) of maternal blood were drawn from each participant via venipuncture at three gestational ages (i.e., 6, 7, and 8 -10 weeks’ gestation). After excluding participants that reported miscarriage during the study, reported twin/triplet pregnancy, or miscalculated gestational age at the time of the initialsample collection, 115 individuals completed the trial in its entirety. Method: Between May and September 2021, 156 pregnant women were recruited from 12 clinics in the United States. The SneakPeek assay was further optimized to enable fetal sex identification at earlier gestations and its performance characteristics were determined at 6-weeks’ gestation. To our knowledge, no prior study has shown fetal sex identification from maternal plasma earlier than seven weeks of gestation with high accuracy. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Ĭlinics Mother Child Health, Vol.19 Iss.4 No:1000419 ABSTRACTīackground: The SneakPeek® Early Gender Test was previously shown to be 99.9% accurate in determining fetal sex as early as seven weeks of gestation. CMCH-22-17127 (R) Published: 0, DOI: 10.35248/2090-7214.22.19.419Ĭitation: Szucs HD, Asprer L, Abunadi N, Milot H, Cacia S, Jacob C (2022)SneakPeek Early Gender Test: The Earliest and Most Accurate Method for Fetal Sex Determination at 6-Weeks Gestation. CMCH-22-17127 Editor assigned: 1, PreQC No. Gateway Genomics, San Diego, California, USAĬorrespondence to: Henriett Diana Szucs, Gateway Genomics, San Diego, California, US, E-mail: 0, Manuscript No. Do it for fun, but ymmv.Henriett Diana Szucs*, Lia Asprer, Nora Abunadi, Haley Milot, Sarah Cacia, Chris Jacob TLDR: the science works, but this company's methodology may not be robust. As a scientist in this field, I can say that performing a test such as this by mail order is much more complicated than if a patient were to come to a testing facility/lab and give a sample under controlled circumstances. So while the science is absolutely sound, this company's execution of the science seems slightly suspect to me. Looking at their website, they do a somewhat poor job of describing their method of testing and their system of controls. They would also describe a limit of detection or a lower limit of resolution for the test. Now a robust test and a reputable lab would have a multitude of controls that would be processed alongside the sample to prevent situations such as these. Even something as simple as the package getting left in the sun and all the reagents getting too hot can negatively impact the test. Wrong ratio of blood to sample/lysis buffer? The DNases present in blood will degrade the DNA over time and your sample will be negative. There is too little fetal DNA in your sample to be amplified and detected? They don't find a Y and assume you are having a girl. They falsely tell you that you have having a boy. Your husband touches the tube? Possible Y contamination. Gender testing specifically is prone to contamination. There are many more variables that can be introduced and therefore lead to potentially inaccurate results. That being said, there is a lot of room for error by sending a patient an at home test. They are specifically working to detect the Y chromosome- if they detect a Y, you are having a boy and if they do not detect a Y, you are having a girl, since as a woman you have 2 X chromosomes. It works by isolating and amplifying the fetal DNA, so it can be detected. It works by detected the free fetal DNA which is circulating in the mother's blood. I'm a scientist who works in molecular diagnostics.
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